O-PTIR Microscope

mIRage-LS

mIRage-LS is a far-field infrared microscope based on O-PTIR (Optical Photothermal Infrared) technology for infrared spectroscopy, hyperspectral mapping and imaging. It allows wavelength independent sub-micrometer lateral resolution for the non-contact measurement in the middle infrared regime (Mid-IR).
It is optimized for the analysis of soft biological samples both hydrated and non-hydrated. Furthermore, the instrument also provides both visible-Raman and fluorescence measurements for co-localized IR, Raman and fluorescence analysis on the same cell or tissue district, with the same lateral resolution and, for IR and Raman measurements, at the same time.

  • O-PTIR spectroscopy in reflection mode, with FTIR transmission-like spectral quality.
  • Point spectroscopy and single line array spectroscopy measurements.
  • IR hyperspectral imaging.
  • IR imaging at a fixed wavenumber(s) of interest.
  • Sub-micron infrared and simultaneous Raman spectroscopy:
    • IR spatial resolution: ≤500 nm in imaging, line scans and single point data collection.
    • Raman spatial resolution: ≤500 nm.
    • O-PTIR measurement time: 1-10 secs for measurements with acceptable Signal to noise
    • Mid-IR laser sources: IR Quantum Cascade Laser (QCL).
    • Visible Probe Laser/Raman Excitation: 532 nm.
    • QCL laser spectral range: 1800-950 cm-1.
    • Raman spectral range: 3900-200 cm-1.
    • Spectral resolution of QCL laser <1 cm-1, typical spectral resolutions used with O-PTIR range from of 2 cm-1 to 4 cm-1 or greater.
    • Objective (Vis/IR/Raman): 40x visible/IR/Raman (0.78 NA, 8.3 mm working distance, reflective Cassegrain); 10x visible (0.30 NA, 16 mm working distance, refractive).
  • Co-localized O-PTIR spectral imaging and epi-fluorescence:
    • Sensor: back illuminated sCMOS monochrome.
    • Resolution: 2048 x 2048.
    • Quantum Efficiency: up to 95%.
    • Spectral Range: 370 – 1100 nm.
  • X-Y Motorized Stage on range 75 mm x 110 mm, with minimum step size of 100 nm.
  • Sample dimensions: accommodate standard (75 x 25 mm) glass slides; up to 60 mm (X,Y) and 22 mm max sample thickness.


📍 The system is located at the SISSI Beamline at Elettra Sincrotrone Trieste in Area Science Park.

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