Fast Protein Liquid Chromatography Systems (FPLC)

ӒKTA pure T 25
ӒKTA pure micro 25
ÄKTA pure T 25 is flexible chromatography system for fast purification of proteins and antibodies from microgram to gram scale. It is suitable for purifying DNA “impurities” in protein samples, DNA, RNA or nucleic acid purification, phenylalanine rich proteins.
ÄKTA pure micro is a chromatography system for high-resolution purification in microliter scale. It is designed for improved resolution and sharper peaks and supporting Cryo-EM purifications.

  • Two high-performance system piston pumps
  • Flow rate setting: 0.001 to 25 mL/min (up to 50 mL/min during column packing)
  • Pressure range: 0 to 20 Mpa
  • Viscosity range: 0.35 to 10 cP (5 cP above 12.5 mL/min)
  • Mixer volume: ÄKTA pure 25: 0.6, 1.4, or 5 mL; ÄKTA pure micro: 0.6 mL
  • Gradient flow rate range: ÄKTA pure 25: 0.1 to 25 mL/min; ÄKTA pure micro: 0.25 to 2 mL/min with 0.6 mL mixer 0.05 to 0.25 mL/min without mixer
  • UV monitor dual wavelenght: LED 280 and 260 nm
  • Resolution: 0.001 mAU
  • Flow rate: 25 mL/min


📍The system is located at Elettra Sincrotrone Trieste laboratories in Area Science Park.


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