Protein Biomarker Analysis System
Olink Signature Q100 is a benchtop solution for multiplex protein detection. It is a dedicated system specifically designed for readout of Olink protein biomarker studies. It is developed for Olink Target (96 & 48-plex), Olink Flex (15–21 chosen assays) and Olink Focus (custom design) protein biomarker panels. These are focused on specific disease areas or biological processes and offer Proximity Extension Assay (PEA) technology coupled to a qPCR readout. PEA combines the best of antibody- and DNA-based methodologies to provide unique, enabling tools for protein biomarker discovery and development. The exponential amplification properties of PCR are utilized in PEA to achieve a strong readout signal, providing assay sensitivity on par or better than traditional enzyme-linked immunosorbent assays (ELISAs).
- Extremely small sample volumes (1 µL) needed to measure large numbers of proteins simultaneously, facilitating studies with limited sample availability.
- Exceptional specificity even at high multiplexing levels.
- Thermal control: Peltier-based, 4–99 ºC.
- Heating ramp rate: up to 5.5 ºC/sec.
- Cooling ramp rate: up to 5.5 ºC/sec.
- Fluorescence excitation: 475 nm, 575 nm.
- Fluorescence emission: 525 nm, 630 nm.
Available panels for Olink Signature
- Olink Target 96: target specific disease areas or key biological processes with multiplex immunoassay panels.
- Olink Target 48: extensively validated and carefully selected biomarkers for a comprehensive view of cytokine signaling and inflammatory processes.
- Olink Flex: run individually tailored studies by combining up to 21 biomarkers from Olink extensive library with minimal sample volume.
- Olink Focus: custom designed panels that help transition from biomarker discovery to clinical utility of protein signatures.
