Orbitrap Exploris™ 120 Mass Spectrometer

This is a hybrid quadrupole Orbitrap mass spectrometer Exploris 120 (Thermo Fisher Scientific) interfaced by a front-end UHPLC system Vanquish Flex. The instrument is capable of 120K resolution and has a 22 Hz scan/speed at 60.000 resolution. Routinary mass accuracy with online calibration is < 1 ppm. Polarity switching is available.
The instrument is capable of different scan modes, Full Scan, SIM. SIM/MS/MS (PRM), DDA, DIA, AIF. HRMS data are analyzed with Compound Discoverer software (Thermo).

Mass range: standard mass range m/z 40–3,000
Orbitrap mass analyzer resolution: up to 120,000 at m/z 200
Scan rate: up to 22 Hz at resolution setting at 15,000
Mass accuracy: external calibration achieves <3 ppm RMS drift over 24 hours; 1-point calibration achieves <3 ppm RMS drift over at least 4 weeks; Internal lock mass calibration achieves <1 ppm RMS drift over 24 hours; EASY-IC achieves <1 ppm RMS drift for at least 5 days
Instrument detection limit: full Scan: < 3.0 fg reserpine on column; tMS2: < 3.0 fg reserpine on column; SIM: < 1.5 fg reserpine on column; Sensitivity MS/MS: 200 fg reserpine on column S/N 100:1; tSIM: 200 fg reserpine on column S/N 250:1.
Dynamic range: >5,000 within a single Orbitrap mass analyzer spectrum
Polarity switching: One Full Scan cycle <700 ms equals >1.4 Hz; One tSIM Scan cycle <600 ms equals >1.6 Hz
Multiplexing: up to 20 precursor ions per scan in tMS2 and up to 20 compounds per scan for tSIM

4 Orbitrap Exploris 120 Mass Spectrometers are available to the users.

📍The instrument is located at DIFARMA of the University of Salerno.

Go Back