Mass Spectrometry Facility

Mass Spectrometry Imaging techniques allows to perform spatial metabolomic analysis to directly assess the spatial distribution of metabolites, lipids, and proteins within tissue, in a "label-free" manner, without the need for homogenization.

Multi-omic Orbitrap-MS Station

The station is capable of both qualitative and quantitative analysis, halving the time needed for analyses. In detail, they will be devoted to i) Untargeted/targeted analysis of polar metabolites by HILIC-HRMS, ii) Untargeted/targeted analysis of lipids by RP-UHPLC-HRMS, iii) DMPK based on MS, iiii) Bottom-up and chemical proteomics to highlight novel druggable proteins by nano-LC-HRMS. These new stations can be run in parallel, with specific workflows for the analyte class, with minimal downtimes, with digital access for remote users.
📍The “Multi-omic Orbitrap-MS station” is located at DIFARMA of the University of Salerno.

High Throughput Sample-Prep Station

This station allows for label-free and robotically automated preparation of samples for the non-destructive analysis of spatial distribution of biomolecules in the target tissue. The employed instruments include liquid handlers, shakers, vacuum-assisted evaporators, and utilize green approaches, such as supercritical fluid extraction (SFE) coupled with supercritical fluid chromatography-MS. This combination ensures rapid analysis, robustness, and minimal environmental impact, particularly for the swift examination of biological specimens like dried blood spots. By automating the process, potential variations introduced by manual handling are reduced, simultaneously enhancing user safety during the manipulation of biological samples.
📍 The “High throughput Sample-Prep station for MS station” is located at DIFARMA of the University of Salerno.

Multimodal Mass Spectrometry Imaging Q-TOFs Station

Featuring an automated sample preparation station, providing high repeatability and safer protocols for operators, this station can perform multimodal Mass Spectrometry Imaging (MSI) and surpass the limitation of the current MSI device by ion mobility technology.
📍 The “Multimodal Mass spectrometry Imaging Q-TOFs station” is located at DIFARMA of the University of Salerno.

Biomedical Accelerator Mass Spectrometry

Biomedical Accelerator Mass Spectrometry (BAMS) is a powerful bio-analytical method in the areas of pharmacology and toxicology and an essential asset for drug-development. Its efficacy lies in the detection of the radioactive isotope of carbon (14C), facilitating a comprehensive understanding of pharmacokinetics and metabolism. BAMS integrates drug discovery and development enabling a variety of biological applications including studies on cell turnover, pharmacokinetics and biodistribution of novel drug candidates and covalent binding of compounds with DNA and proteins. Thanks to the sensitivity and samples processing capabilities, it allows more cost and time effective drug development through microdosing and microtracing.
📍 The “Biomedical Accelerator Mass Spectrometry station” is located at the University of Salento.

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