Orbitrap Fusion Lumos Tribrid Mass Spectrometer

Orbitrap Fusion Lumos Tribrid Mass Spectrometer

The instrumentation is an Ultimate 3000 nano-UHPLC coupled to Orbitrap Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific) for both bottom-up (analysis of peptides) and top-down (intact protein) proteomics, allowing for unparalleled resolution and accuracy in protein profiling. High resolution mass spectrometry is now the workhorse of proteomics, to identify and quantify proteins in complex and precious samples, such as cell lysates, organoids, biofluids and biopsies high sensitivity and fast scanning speed are necessaries. Orbitrap mass spectrometers series, with their ultra-high resolution and excellent mass accuracy are highly suited for both bottom-up and top-down proteomics, enabling to identify and quantify thousands of proteins in a single run, helping in evaluate protein turnover and modulation in disease models, as well as to study the effect of novel drugs. The present instrument is a hybrid quadrupole-Orbitrap-Ion trap MS platform dedicated to proteomic analysis by both labelling or label free approaches.

  • Coupled to nano UHPLC Ultimate 3000
  • ESI and nESI interface
  • External and internal calibration possible
  • Max Resolution: 1.000.000
  • MS/MS modes: DDA, DIA, PRM, SIM
  • CID, HCD, ETD fragmentation
  • Analyzer: Ion Trap and Orbitrap
  • Mass accuracy <1 ppm
  • Software: Proteome Discoverer for alignment, annotation, and filtering; Freestyle for raw data analysis

The instrument is available to users through CERIC-ERIC and is part of the “BOL: Bio Open Lab” project funded by the Ministry of University and Research (Research and Innovation PON 2014-2020 program).

📍 The instrument is located at DIFARMA of the University of Salerno.

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