Proteomics Analysis

Cutting-edge technology and expertise to provide comprehensive insights into protein dynamics, interactions, and modifications.
Depending on the protein of interest, our sample preparation protocols can be either manual or automated (using systems like PreOmics-PreON), completed in as little as 3 hours. This includes cell lysis, trypsin digestion of proteins into peptides, and subsequent peptide fragmentation.

Peptides are analysed using nano liquid chromatography coupled to high resolution tandem mass spectroscopy, followed by comparison against protein sequence databases like UniProt to ensure accurate protein identification and characterization.

The instrumentation employed for proteomics analysis is an ultimate 3000 nano-UHPLC coupled to Orbitrap Lumos tribrid MS Thermo Fisher Scientific for both bottom-up (analysis of peptides) and top-down (intact protein) proteomics, allowing for unparalleled resolution and accuracy in protein profiling.

📍The proteomics analysis services are provided by University of Salerno.

Epiproteomics Analysis

Analysis of histone modifications to study how chromatin architecture influences gene expression. This includes the examination of histone deacetylases (HDACs), crucial for the activation or repression of specific cellular processes. We provide support for pharmaceutical chemists focused on elucidating drug mechanisms of action, particularly how therapeutic agents interact with proteins and modify their function.

Post-Translational Modifications (PTM) Identification

Detailed analysis of protein modifications such as methylation, phosphorylation, and acylation, focusing on their roles in regulating cellular processes and disease mechanisms.

  • Site-specific modification identification: we provide precise identification of modified amino acid residues, enabling a deeper understanding of the structural and functional implications of PTMs. This insight is crucial for both basic biological research and the development of therapeutic strategies.
  • Supporting structural analysis: our capabilities extend to structural biology, assisting in the interpretation of how modifications affect protein structure and function at a molecular level.

Analysis of Low-Concentration Protein Biomarkers

Detecting molecular alterations in proteins that are present only in small amounts, such as cancer markers, in various types of samples including plasma, cells, and other biological specimens, including FFPE (Formalin-Fixed Paraffin-Embedded) tissues. Identifying low-abundance protein changes is pivotal for early diagnosis and monitoring of therapeutic responses.

Selected Publications

  • “Link between organic nanovescicles from vegetable kingdom and human cell physiology: intracellular calcium signalling”
    M. Trentini, Journal of Nanobiotechnology, 2024, 22, 68
    DOI: 10.1186/s12951-024-02340-8
  • “Multi-omics analysis reveals attenuation of cellular stress by empagliflozin in high glucose-treated human cardiomyocytes”
    L. Scisciola et al., Journal of Translational Medicine, 2023, 21, 662
    DOI: 10.1186/s12967-023-04537-1